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Platelet Immunology Laboratory

Platelet Autoantibody Test

  • Test Code: 3640-00
  • CPT Codes: (86022/86023)x2, 83912

Idiopathic Thrombocytopenic Purpura (ITP) is a diagnosis associated with elevated platelet associated IgG, IgM or both. Positive autoantibody test results are indicative of autoimmune destruction. Positive indirect antibody test results, using patient's plasma against normal random platelets, may indicate ITP depending upon the patient's transfusion or pregnancy history. In approximately 85% of patients with ITP the presence of an autoantibody can be detected.

Patients who require autoantibody testing must be platelet transfusion independent for approximately 5 days to ensure that the platelets being tested are the patient's own platelets and not circulating donor platelets.

  • Tests Performed: Flow Cytometry
  • Test Types: Direct and Indirect
  • Samples Required: 5 citrate tube tube (blue top) and EDTA (lavender top) as below:
Platelet Count Volume in EDTA
Greater or equal to 50,000
20cc
20,000 to 49,999 30cc
10,000 to 20,000 40cc
Less than 10,000 DO NOT DRAW
We cannot recover sufficient platelets to perform a direct assay ofantibody.. bound to autologous platelets. Contact Lab Supervisor for testing options.
  • Analytic Time: 1 Day
  • Send whole blood, room temperature
  • Refer to Ordering Information page for submission and transporting requirements
  For questions call the laboratory: (206) 292-6543