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Red Cell Reference Laboratory

Kleihauer-Betke Stain

  • Transaction Code: 3146-00
  • CPT Code (Suggested): 85460

The Kleihauer-Betke test is a quantitative assay for fetomaternal hemorrhage that detects fetal red blood cells in the presence of maternal red blood cells by staining of a blood smear following acid elution. It is based on the relative resistance of fetal hemoglobin (HbF) for acid treatment compared to maternal adult hemoglobin (HbA). The results are used to calculate the dose of Rh immune globulin (RhIg) to administer to an Rh negative woman for post-natal prophylaxis of Rh alloimmunization.

  • Sample Required: 7 ml EDTA
    (If postnatal sample, draw > 1 hour after delivery)
  • Normal Test Values: The percentage of fetal hemoglobin in adults is normally < 1%
  • Analytic Time: 24 hours if weekday; 48 hours if weekend
  • Days Test is Set Up: Monday through Friday
  For questions call the laboratory: (206) 292-6534