Mass Spectrometry Lab
Our Mission at the Mass Spectrometry Laboratory at Puget Sound Blood Center is to aid researchers in their studies using mass spectrometry and proteomics. With our state-of-the-art facility we are now able to provide analysis, training, and consultation to investigators within the Blood Center and external collaborators. Our service includes:
Thermo-Finnigan LCQ Deca XP Plus mass spectrometer
Agilent 1100 HPLC system interfaced to a Thermo-finnigan LCQ Deca XP Plus MS detector via an electrospray ionization source. The HPLC system is equipped with a narrow bore reverse phase C18 column for routine analysis of peptides, small proteins and metabolites mixtures. The instrument's scanning range is 200-2000 m/z. Generally we request ~25 pmol of sample for analysis. However, if method development for your sample is necessary, we will need more depending on your project. We prefer to have the sample (peptides or proteins) at a concentration of 1-5 µM.
Thermo-Finnigan LTQ Orbitrap Velos hybrid FT mass spectrometer
Thermo linear ion-trap (LTQ) Orbitrap Velos Mass spectrometer provides ultra-high resolution and accurate mass for confident structural identification as well as molecular weight determination for intact proteins. This instrument is equipped with multiple fragmentation techniques including collision-induced dissociation (CID), Higher-Energy Collisional Dissociation (HCD), and Electron Transfer Dissociation (ETD) for Tandem Mass Spectrometry (MS/MS) analysis. Importantly CID and ETD generate complementary MS/MS spectra for confident identification of both post-translational modifications and peptide sequence. Samples can be introduced directly into the Electrospray ionization (ESI) or nanoESI source or coupled with Waters nanoAcquity ultra performance liquid chromatography (UPLC) system to perform nanoLC-MS/MS analyses for digestion mixture or complex samples. Generally we request 5 pmol of protein of interest or at least 20 μg total proteins of cell lysate or biological samples for analysis. However, if extensive method development is required we may request more.