Puget Sound Blood Center | Research: Reems Lab

Griep, M. A., Reems, J. A., Franden, M. A., and McHenry, C. S., (1990) Reduction of the Potent DNA Polymerase III Holoenzyme 3'—>5' Exonuclease Activity by Template-Primer Analogues. Biochemistry 29: 9006-9014.

Reems, J. A., Griep, M. A., and McHenry, C. S., (1991) The Proofreading Activity of DNA Polymerase III Responds Like the Elongation Activity to Auxiliary Subunits. J. Biol. Chem. 266: 4878-4882.

Wu, A. C., Zechner, E. L., Reems, J. A., McHenry, C. S., Marians, K. J. (1992) Coordinated Leading- and Lagging- Strand Synthesis of the E. coli DNA Replication Fork V. Primase Action Regulates the Size of Okazaki Fragment Synthesis. J.Biol. Chem., 267: 4074-4083.

Reems, J. A., and McHenry, C. S., (1994) E. coli DNA Polymerase III Holoenzyme Protects 3 Helical Repeats of Primer-Template. J. Biol. Chem. 269: 33091-33096.

Reems, J. A., Wood, S., and McHenry, C. S. (1995) E. coli DNA Polymerase III Holoenzyme Subunits, a, b, and g Directly Interact with Primer-Template. J. Biol. Chem. 270: 5606-5613.

Reems, J. A., and Torok-Storb, B. (1995) Cell-Cycle and Functional Analysis of in vitro Stimulated Human CD34+/CD38lo and CD34+/CD38hi Hematopoietic Progenitor Cells. Blood 85: 1480-1487.

Kirk, J. A., Reems, J. A., Roecklein, B. A., Van Devanter, D. R., Bryant, E. M. Radich, J., Edmands, S., Lee, A., and Torok-Storb, B. (1995) Benign Marrow Progenitors are Enriched in the CD34+/HLA-DRlo Population but not in the CD34+/CD38lo Population in Chronic Myeloid Leukemia: An Analysis using Interphase Fluorescence in situ Hybridization. Blood 86:737-743.

Kirk, J. A., Radich, J., Edmands, S., Lee, A., Van Devanter, D. R., Reems, J. A., Bryant, E. M. (1996) Unusual Expression of mRNA Typical of Philadelphia Positive Acute Lymphoblastic Leukemia Detected in Chronic Myeloid Leukemia. American Journal of Hematology 52:129-134.

Mielcarek, M., Reems, J. A., Torok-Storb, B. (1997). Extrinsic Control of Stem Cell Fate: Practical Considerations. Stem Cells 15 (suppl 1):229-236

Reems, J. A., Mielcarek, M., Torok-Storb, B. (1997) Differential Modulation of Adhesion Markers with Ex Vivo Expansion of Human Umbilical CD34+ Cells. Biol Blood and Marrow Transplant 3:133-144.

Roecklein, B.A., Reems, J. A., Rowley, S., Torok-Storb, B (1998) Ex vivo Expansion of Immature 4-Hydroxycyclophosphamide Resistant Progenitor Cells from G-CSF Mobilized Peripheral Blood. Biol of Blood and Marrow Transplant 4:61-8.

Reems, J. A., Fujita, D.T., Tyler, T., Moldwin, R. L., Smith, S.D. (1999) Obtaining an Accepted Investigational New Drug (IND) Application to Operate an Umbilical Cord Blood Bank. Transfusion 39:357-363.

Xu, R., Medchill, M., Reems, J. A. (2000) Serum Supplements, Inoculum Density and Accessory Cell Effects are Dependent upon the Cytokine Combination Selected to Ex Vivo Expand Human Hematopoietic Stem/Progenitor Cells. Transfusion 40:1299-1307.

Xu, R., Reems, J. A. (2001) Umbilical Cord Blood Progeny Cells Expressing the CD34+ Phenotype Post-Expansion have Reduced Engraftment Potential Relative to Unexpanded CD34+ Cells. Transfusion 41:213-218.

Sweet, IR, Kalil G, Wallen A.R., Steedman M, Schenkman, K.A., Reems, J. A., Kahn, S.E., Callis, J.B. (2002) Continuous measurement of oxygen consumption by pancreatic islets. Diabetes Technol Ther 5:661-672.

Matsumoto, S., Qualley, S., Goel, S., Hagman, D., Sweet, I., Poitout, V., Strong, M.D., Robertson, R.P., Reems, J.A. (2002) Effect of the two-layer [University of Wisconsin solution/perfluorochemical plus O2] method of pancreas preservation as assessed by the Edmonton isolation protocol. Transplantation 74:1414-1419.

Matsumoto, S., Rigley, T., Qualley, S., Kuroda, Y., Reems, J. A., Stevens, R.B. (2002) Efficacy of the oxygen-charged static two-layer method for short-term pancreas preservation and islet isolation from non-human primate and human pancreata. Cell Transplantation. 11:769-777.

Matsumoto, S., Rigley, T., Reems, J. A., Kuroda, Y., Stevens, B.R. (2003) Improved islet yields from Macaca Nemestrina and marginal human pancreata after two-layer method preservation and endogenous trypsin inhibition. American Journal of Transplantation 3:53-63.

Newman, H., Reems, J. A., Rigley, T.H., Bravo, D., Strong, D.M. (2003) Donor age and gender are the strongest predictors of marrow recovery from cadaveric vertebral bodies. Cell Transplantation. 12:83-90.

Matsumoto, S., Goel S., Qualley S., Strong, D.M., Reems, J.A., (2003) A comparative evaluation of culture conditions for short-term maintenance (<24hr) of human islets isolated using the Edmonton protocol. Cell & Tissue Banking 4:85-93.

Lannutti, B.L., Shim, M-H., Reems, J.A., Drachman J.G., (2003) Identification and Activation of Src Family Kinases in Primary Megakaryoyctes. Exp. Hematology 31:1268-1274.
Wada, R.K., Bradford, A., Moogk, M., Yim R., Strong D.M., Drachman J., Reems, J.A. (2004) Cord Blood Units Collected at a Remote Site: A Collaborative Endeavor to Collect Umbilical Cord Blood Through the Hawaii Cord Blood Bank and Store the Units at the Puget Sound Blood Center. Transfusion 44:111-118.

Sweet I.R, Cook D.L., DeJulio E., Wallen A.R., Khalil G., Callis J.B., Reems JA: (2004) Regulation of ATP/ADP in pancreatic islets. Diabetes 53:401-409.

Zhang, G., Matsumoto, S., Hyon, S-H., Reems, J.A. (2004) Polyphenol, an Extract of Green Tea, Improves Culture Recovery Rates of Marginal Grade Human Islets by Precluding apoptosis. Cell Transplantation 13:145-152.

Charpentier, K. P., Levy A., Davis, C., Robertson R. P., Reems, J.A., Marsh, C., (2004) Acalculous Cholecystitis Complicating Intra-Portal Islet Embolization for Simultaneous Kidney/Islet Transplantation. Surgical Rounds 27:335-337.

Matsumoto S., Zhang G., Qualley S., Clever J., Tombrello, Y., Strong D. M., Reems J.A. (2004) The Effect of the Two-Layer (University of Wisconsin Solution/Perfluorochemical) Preservation Method on Clinical Grade Pancreata Prior to Islet Isolation and Transplantation. Transplantation Proceedings 36: 1037-1039.

Matsumoto S., Zhang G., Qualley S., Clever J., Tombrello, Y., Strong D. M., Reems J.A. (2004) Analysis of Donor Factors Affecting human Islet Isolation with Current Isolation Protocol. Transplantation Proceedings 36:1034-1036.

Shim, M.H., Hoover, A., Blake, N., Drachman, J.G., Reems, J.A., (2004) Gene Expression Profile of Primary Human CD34+/CD38lo Cells Differentiating Along the Megakaryocyte Lineage. Exp. Hematol 32:638-648.

Matsumoto S., Tanaka K., D. Strong D. M., Reems, J.A. (2004) Efficacy of Human Islet Isolation from the Tail Portion of Pancreas for the Possibility of Living Donor Islet Transplantation. Transplantation 78:839-843.

Lannutti BJ, Blake N, Gandhi MJ, Reems JA, Drachman JG. (2005) Induction of polyploidization in leukemic cell lines and primary bone marrow by Src kinase inhibitor SU6656. Blood 105:3875-3878.

Wang W, Upshaw L, Strong DM, Reems, JA. (2005) Increased oxygen consumption rates in response to high glucose detected by a novel oxygen biosensor system in non-human primate and human islets. J. Endocrinology 185:445-455.

Matsumoto S, Iwanaga Y, Okitsu T, Noguchi H, Yonekawa Y, Tanaka K, Strong DM, Reems JA, Gaur LK. (2005) Analysis of large-scale nonhuman primate islet isolations. Transplant Proc. 37:1317-21.
Ghandhi MJ, Drachman JG, Reems JA, Thorning D, Lannutti BJ. (2005) A Novel Strategy for Generating Platelet-Like Fragments from Megakaryocytic Cell Lines and Human Progenitor Cells. Blood Cells Mol Dis 35:70-73.

Sweet IR, Gilbert M, Jensen R, Sabek O, Fraga DW, Gaber AO, Reems JA. (2005) Glucose stimulation of cytochrome C reduction and oxygen consumption as assessment of human islet quality. Transplantation 80:1003-1011.

Moroff G, Eichler H, Brand, A, Kekomaki R, Kurtz, J, Letowska, M, Pamphilon, D, Read, EJ, Porretti L, Lecchi L, Reems JA, Racher R, Seetharaman S, Takahashi TA. (2006) Multiple-Laboratory comparison of in vitro Assays Utilized to Characterize Hematopoietic Cells in Cord Blood. Transfusion 46:507-515

Shapiro AM, Ricordi C, Hering BJ, Auchincloss H, Lindblad R, Robertson RP, Secchi A, Brendel MD, Berney T, Brennan DC, Cagliero E, Alejandro R, Ryan EA, DiMercurio B, Morel P, Polonsky KS, Reems JA, Bretzel RG, Bertuzzi F, Froud T, Kandaswamy R, Sutherland DER, Esenbarth G, Segal M, Preksaitis J, Korbutt GS, Barton FB, Viviano L, Seyfert-Margolis V, Bluestone J, Lakey JRT. (submitted) International Multicenter Trial of Islet Transplantation with the Edmonton Protocol in Type I Diabetes. N. Eng. J Med.